E coli - Disease of the Day - Custom Scholars
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E coli – Disease of the Day

question
E coli is...
answer
gram negative bacilli
-rod shaped
-can grow without oxygen
-has flagella
question
E. coli O157:H7
answer
- emerging cause of food bourne illnesses
-strain of e coli that was originally isolated from Argentina
- cause of illness is severe bloody diarrhea
-"hamburger disease"
question
O and H
answer
O- antigen LPS
H - refers to the flagella antigen
question
Main Problem of Shiga Toxin Bacteria
answer
- produces shiga like toxins
-lines intestines and destroys healthy cells
- escapes into blood stream and kills erythrocytes
Shiga Toxins can target ribosomes and inhibit protein production
- e coli acquires genetic code from horizontal gene transfer
-antibiotic treatment would allow for the shiga toxin to acquire code!
question
Symptoms/ Signs
answer
-abdominal cramps and headaches
-progress of bloody diarrhea
-fever and vomiting
-destruction of kidneys
question
Diagnosis
answer
isolation of e coli in a stool sample with MacConkey agar - inhibits the growth of gram (+)
-ferments sorbitol to make acid
-petree dish will change color with presence of acid
question
Source of the E coli
answer
cattle, and intestines of sick people
-90% of meat is contaminated
-hamburgers especially!!
question
Prevention
answer
-cooke meat thoroughly
-avoid spreading harmful bacteria in kitchen
1 of 8
question
E coli is...
answer
gram negative bacilli
-rod shaped
-can grow without oxygen
-has flagella
question
E. coli O157:H7
answer
- emerging cause of food bourne illnesses
-strain of e coli that was originally isolated from Argentina
- cause of illness is severe bloody diarrhea
-"hamburger disease"
question
O and H
answer
O- antigen LPS
H - refers to the flagella antigen
question
Main Problem of Shiga Toxin Bacteria
answer
- produces shiga like toxins
-lines intestines and destroys healthy cells
- escapes into blood stream and kills erythrocytes
Shiga Toxins can target ribosomes and inhibit protein production
- e coli acquires genetic code from horizontal gene transfer
-antibiotic treatment would allow for the shiga toxin to acquire code!
question
Symptoms/ Signs
answer
-abdominal cramps and headaches
-progress of bloody diarrhea
-fever and vomiting
-destruction of kidneys
question
Diagnosis
answer
isolation of e coli in a stool sample with MacConkey agar - inhibits the growth of gram (+)
-ferments sorbitol to make acid
-petree dish will change color with presence of acid
question
Source of the E coli
answer
cattle, and intestines of sick people
-90% of meat is contaminated
-hamburgers especially!!
question
Prevention
answer
-cooke meat thoroughly
-avoid spreading harmful bacteria in kitchen

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