Introductory Microbiology 220 - Custom Scholars
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Introductory Microbiology 220

question
Complete Hemolysis on blood agar is called.
answer
Beta
question
Which type of media is NOT selective.
answer
Blood agar
question
MacConkey (MAC) agar is enriched with salt.
answer
FALSE
question
An ____________ medium contains one or more compounds to distinguish between microbes in the medium, while an __________________ medium contains one or more additiatives to encourage the growth of fastidious microbes.
answer
Differential and enriched.
question
Mannitol salt agar (MSA) is selective for ____________ and differential for ____________.
answer
Salt tolerant organisms, mannitol fermentation.
question
MacConkey agar is differential for ________ fermentation.
answer
Lactose
question
Which of the following types of agar would be the most helpful for isolating and differentiating Staphylococcus aureus from others types of organisms.
answer
Mannitol salt agar
question
Blood agar plates are ________ for hemolysis.
answer
Differential
question
Phenylethanol agar is used ____________.
answer
To select for gram positive bacteria
question
Eosin Methylene Blue (EMB) agar is selective for ___________ and differential for ___________.
answer
Gram Negative organisms and lactose fermentation.
question
Name one disease that streptococcus pyogenes can cause
answer
Strep throat, scarlet fever, gangrene, ear infection, or flesh eating disease
question
In lab, we tested streptococci groups A-D for susceptibility to
answer
Bacitracin
question
Name one disease that streptococcus pneumoniae can cause
answer
Pneumonia, ear infection, meningitis, or conjunctivitis
question
How does beta hemolysis appear on blood agar?
answer
A clear zone surrounding the colony
question
The enzyme phenylalanase converts phenylalanine into
answer
Ammonia and Phyenylpyruvic acid
question
Which of the following organisms are never part of the normal flora of the skin, mucous membranes, and throat?
answer
Staphylococcus saprophyticus
question
T or F: Shigella can cause typhoid fever
answer
False
question
Which of the following is part of the definition for a coliform?
answer
Facultative anaerobes, Ferments lactose to acid and gas, and Gram negative
question
T or F: The only member in Group B streptococcus is streptococcus pyogenes
answer
False
question
Staphylococci are _____ while streptococci are ______
answer
Catalase positive; catalase negative
question
Which of the following tests did you perform on your Enterobacteriaceae Unknown last period
answer
Citrate, and SIM
question
Which group of streptococci is responsible for the formation of dental plaque?
answer
Viridans group
question
Which of the following organisms is a member of Group C streptococcus
answer
Streptococcus equi
question
Which of the following tests did you perform on your staphylococcus unknown last period?
answer
Coagulase
question
The reagent used to test for phenylalanine deamination is _______ The reagent turns _______ as a positive result
answer
Ferric chloride, green
question
What is the term that is used if water is coliform free and drinkable?
answer
Potable
question
A negative test for the utilization of citrate would result in ________
answer
A green color
question
Which type(s) of media did you use to isolate your unknown organisms?
answer
Mannitol salt agar (MSA), and MacConkey agar (MAC)
question
T or F

To test for starch hydrolysis, the reagent iodine was added to the plate AFTER incubation
answer
True
question
Which of the following organisms is used as an indicator of fecal contamination of water?
answer
Escherichia coli
question
Name one illness caused by staphylococcus saprophyticus
answer
UTI (in Females)
question
Which of the following would be a positive result for the production of the enzyme coagulase?
answer
The formation of a clot in the test tube
question
T or F: The rapid staphylococcus aureus test is based on antigen/ antibody reactions
answer
True
question
Which of the following organisms is resistant to the antibiotic novobiocin?
answer
Staphylococcus saprophyticus
question
Which of the following organisms' ferments lactose?
answer
Escherichia coli
question
What would a positive test for the rapid detection of Streptococcus pyogenes look like?
answer
Two lines
question
Which of the following organisms causes hemolysis on blood agar?
answer
Staphylococcus aureus
question
T or F: When trying to identify a bacterium as a member of streptococcus Group A, B, C, or D, testing for susceptibility to the antibiotic bacitracin will give you sufficient information to identify the organism
answer
False
question
Which of the following organisms utilizes citrate?
answer
Salmonella
question
Which of the following organisms produces tryptophanase?
answer
Escherichia coli
question
Suppose you are given an unknown sample that you need to identify as either salmonella or shigella. To accomplish this, you decide to use the rapid ID test. When you added the shigella antiserum to your sample, white clumps were produced while the salmonella antiserum resulted in a cloudy sample with no clumps. Based on these results, what was the identity of your unknown?
answer
Shigella
question
Carbohydrate fermentation produces-
answer
May produce Gas and acids
question
Amylase is the enzyme responsible for digesting
answer
starch
question
A positive catalase results in
answer
Bubbles
question
The enzyme unrease converts urea into ammonia and
answer
co2
question
What color will a lactose fermenter appear on MAC
answer
Pink
question
A Durham tube is sued to detact for ___________ produced by carbohydrate fermentation
answer
Gasses or CO2
question
A positive indole test indicates the enzyme _________ was produced by the bacterial strain.
answer
Tryptophanase
1 of 48
question
Complete Hemolysis on blood agar is called.
answer
Beta
question
Which type of media is NOT selective.
answer
Blood agar
question
MacConkey (MAC) agar is enriched with salt.
answer
FALSE
question
An ____________ medium contains one or more compounds to distinguish between microbes in the medium, while an __________________ medium contains one or more additiatives to encourage the growth of fastidious microbes.
answer
Differential and enriched.
question
Mannitol salt agar (MSA) is selective for ____________ and differential for ____________.
answer
Salt tolerant organisms, mannitol fermentation.
question
MacConkey agar is differential for ________ fermentation.
answer
Lactose
question
Which of the following types of agar would be the most helpful for isolating and differentiating Staphylococcus aureus from others types of organisms.
answer
Mannitol salt agar
question
Blood agar plates are ________ for hemolysis.
answer
Differential
question
Phenylethanol agar is used ____________.
answer
To select for gram positive bacteria
question
Eosin Methylene Blue (EMB) agar is selective for ___________ and differential for ___________.
answer
Gram Negative organisms and lactose fermentation.
question
Name one disease that streptococcus pyogenes can cause
answer
Strep throat, scarlet fever, gangrene, ear infection, or flesh eating disease
question
In lab, we tested streptococci groups A-D for susceptibility to
answer
Bacitracin
question
Name one disease that streptococcus pneumoniae can cause
answer
Pneumonia, ear infection, meningitis, or conjunctivitis
question
How does beta hemolysis appear on blood agar?
answer
A clear zone surrounding the colony
question
The enzyme phenylalanase converts phenylalanine into
answer
Ammonia and Phyenylpyruvic acid
question
Which of the following organisms are never part of the normal flora of the skin, mucous membranes, and throat?
answer
Staphylococcus saprophyticus
question
T or F: Shigella can cause typhoid fever
answer
False
question
Which of the following is part of the definition for a coliform?
answer
Facultative anaerobes, Ferments lactose to acid and gas, and Gram negative
question
T or F: The only member in Group B streptococcus is streptococcus pyogenes
answer
False
question
Staphylococci are _____ while streptococci are ______
answer
Catalase positive; catalase negative
question
Which of the following tests did you perform on your Enterobacteriaceae Unknown last period
answer
Citrate, and SIM
question
Which group of streptococci is responsible for the formation of dental plaque?
answer
Viridans group
question
Which of the following organisms is a member of Group C streptococcus
answer
Streptococcus equi
question
Which of the following tests did you perform on your staphylococcus unknown last period?
answer
Coagulase
question
The reagent used to test for phenylalanine deamination is _______ The reagent turns _______ as a positive result
answer
Ferric chloride, green
question
What is the term that is used if water is coliform free and drinkable?
answer
Potable
question
A negative test for the utilization of citrate would result in ________
answer
A green color
question
Which type(s) of media did you use to isolate your unknown organisms?
answer
Mannitol salt agar (MSA), and MacConkey agar (MAC)
question
T or F

To test for starch hydrolysis, the reagent iodine was added to the plate AFTER incubation
answer
True
question
Which of the following organisms is used as an indicator of fecal contamination of water?
answer
Escherichia coli
question
Name one illness caused by staphylococcus saprophyticus
answer
UTI (in Females)
question
Which of the following would be a positive result for the production of the enzyme coagulase?
answer
The formation of a clot in the test tube
question
T or F: The rapid staphylococcus aureus test is based on antigen/ antibody reactions
answer
True
question
Which of the following organisms is resistant to the antibiotic novobiocin?
answer
Staphylococcus saprophyticus
question
Which of the following organisms' ferments lactose?
answer
Escherichia coli
question
What would a positive test for the rapid detection of Streptococcus pyogenes look like?
answer
Two lines
question
Which of the following organisms causes hemolysis on blood agar?
answer
Staphylococcus aureus
question
T or F: When trying to identify a bacterium as a member of streptococcus Group A, B, C, or D, testing for susceptibility to the antibiotic bacitracin will give you sufficient information to identify the organism
answer
False
question
Which of the following organisms utilizes citrate?
answer
Salmonella
question
Which of the following organisms produces tryptophanase?
answer
Escherichia coli
question
Suppose you are given an unknown sample that you need to identify as either salmonella or shigella. To accomplish this, you decide to use the rapid ID test. When you added the shigella antiserum to your sample, white clumps were produced while the salmonella antiserum resulted in a cloudy sample with no clumps. Based on these results, what was the identity of your unknown?
answer
Shigella
question
Carbohydrate fermentation produces-
answer
May produce Gas and acids
question
Amylase is the enzyme responsible for digesting
answer
starch
question
A positive catalase results in
answer
Bubbles
question
The enzyme unrease converts urea into ammonia and
answer
co2
question
What color will a lactose fermenter appear on MAC
answer
Pink
question
A Durham tube is sued to detact for ___________ produced by carbohydrate fermentation
answer
Gasses or CO2
question
A positive indole test indicates the enzyme _________ was produced by the bacterial strain.
answer
Tryptophanase
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