Micro Lab 4 - Custom Scholars
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Micro Lab 4

question
Normal Microbiota of the Skin
answer
Staphylococcus aureus,
Staphylococcus epidermidis and Staphylococcus saprophyticus
question
Staphylococcus aureus
answer
Most pathogenic, Produce and secrete several toxins and enzymes that act on host
immune system to mediate cell destruction
question
Staphylococcus aureus normal microbiota
answer
Skin surface, upper respiratory tract,
nares, axilla, groin, and perineum; can colonize various
epithelial and mucosal membranes
question
Staphylococcus epidermidis
answer
member of normal microbiota and often
encountered in the lab as a contaminant, Important nosocomial pathogen
question
Staphylococcus epidermidis normal microbiota
answer
skin and mucous membranes; distributed
widely over body surface
question
Staphylococcus saprophyticus
answer
2nd most common cause of
uncomplicated UTIs in women, Cause urinary tract infections
question
Staphylococcus saprophyticus normal microbiota
answer
skin and mucosa of the urogenital tract
question
Catalase Test
answer
detects the presence of the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide to H20 and O2
question
Catalase test reagent
answer
hydrogen peroxide (H2O2)
question
positive catalase test
answer
bubbles, catalase is present
question
negative catalase test
answer
no bubbles, catalase not present
question
Catalase
answer
Enzyme that converts Hydrogen Peroxide into Water and Gaseous Oxygen.
question
Positive catalase organisms
answer
Staphylococcus epidermidis, Staphylococcus aureus
question
Negative catalase organisms
answer
enterococcus faecalis, strep
question
Mannitol Salt Agar
answer
-selective and differential
- Isolating Staphylococcus aureus from other staphylococci
question
Growth (-) Mannitol N/A
answer
Organism is inhibited by high level of salt in medium
Not Staphylococcus
question
Growth (+) Mannitol (-)
answer
growing but not fermenting mannitol + not producing acid
A type of Staphylococcus (ex: Staphylococcus epidermidis
question
Growth (+) Mannitol (+)
answer
growing, is fermenting mannitol, producing acid, and changing color of the pH indicator
Staphylococcus aureus
question
Coagulase test
answer
- determine the presence or absence of the coagulase protein.
- Used to differentiate
S. aureus (Positive) from
other Staph spp. (Negative)
question
bound coagulase
answer
"clumping factor" remains attached to the cell surface
question
Free coagulase
answer
secreted by the bacteria I only detected by tube method
question
Staph latex test
answer
rapid test to identify S. aureus
question
Staph Latex Positive
answer
- red agglutination w/ background changed to blue
- control latex has no change, stays purple
- S. aureus
question
Staph Latex Negative
answer
- test latex has no change, stays purple
- control latex has no change as well
question
Sepsis
answer
body's inflammatory response to infection, in which there is fever, elevated heart and respiratory rate, and low blood pressure
1 of 25
question
Normal Microbiota of the Skin
answer
Staphylococcus aureus,
Staphylococcus epidermidis and Staphylococcus saprophyticus
question
Staphylococcus aureus
answer
Most pathogenic, Produce and secrete several toxins and enzymes that act on host
immune system to mediate cell destruction
question
Staphylococcus aureus normal microbiota
answer
Skin surface, upper respiratory tract,
nares, axilla, groin, and perineum; can colonize various
epithelial and mucosal membranes
question
Staphylococcus epidermidis
answer
member of normal microbiota and often
encountered in the lab as a contaminant, Important nosocomial pathogen
question
Staphylococcus epidermidis normal microbiota
answer
skin and mucous membranes; distributed
widely over body surface
question
Staphylococcus saprophyticus
answer
2nd most common cause of
uncomplicated UTIs in women, Cause urinary tract infections
question
Staphylococcus saprophyticus normal microbiota
answer
skin and mucosa of the urogenital tract
question
Catalase Test
answer
detects the presence of the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide to H20 and O2
question
Catalase test reagent
answer
hydrogen peroxide (H2O2)
question
positive catalase test
answer
bubbles, catalase is present
question
negative catalase test
answer
no bubbles, catalase not present
question
Catalase
answer
Enzyme that converts Hydrogen Peroxide into Water and Gaseous Oxygen.
question
Positive catalase organisms
answer
Staphylococcus epidermidis, Staphylococcus aureus
question
Negative catalase organisms
answer
enterococcus faecalis, strep
question
Mannitol Salt Agar
answer
-selective and differential
- Isolating Staphylococcus aureus from other staphylococci
question
Growth (-) Mannitol N/A
answer
Organism is inhibited by high level of salt in medium
Not Staphylococcus
question
Growth (+) Mannitol (-)
answer
growing but not fermenting mannitol + not producing acid
A type of Staphylococcus (ex: Staphylococcus epidermidis
question
Growth (+) Mannitol (+)
answer
growing, is fermenting mannitol, producing acid, and changing color of the pH indicator
Staphylococcus aureus
question
Coagulase test
answer
- determine the presence or absence of the coagulase protein.
- Used to differentiate
S. aureus (Positive) from
other Staph spp. (Negative)
question
bound coagulase
answer
"clumping factor" remains attached to the cell surface
question
Free coagulase
answer
secreted by the bacteria I only detected by tube method
question
Staph latex test
answer
rapid test to identify S. aureus
question
Staph Latex Positive
answer
- red agglutination w/ background changed to blue
- control latex has no change, stays purple
- S. aureus
question
Staph Latex Negative
answer
- test latex has no change, stays purple
- control latex has no change as well
question
Sepsis
answer
body's inflammatory response to infection, in which there is fever, elevated heart and respiratory rate, and low blood pressure
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