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Micro Midterm

question
What is the purpose of BHI with 6.5% NaCl
answer
Differentiates microorganisms based on their ability to grow in 6.5% NaCl
question
What is the purpose of bile esculin hydrolysis?
answer
Differentiates mnicroorganisms based on their ability to hydrolyze esculin to esculetin and dextrose. The esculetin reacts with ferric citrate in the medium to dorm a dark-brown complex.
question
What is catalase purpose?
answer
Differentiates microorganisms based on their ability to produce the catalase enzyme. Catalase breaks hydrogen peroxide down to water and oxygen.
question
What is coagulase's purpose?
answer

Detection of coagulase that is excreted by Staphylococcus aureus that causes fibrin clot formation.

question
What are the three types of hemolysis on SBA?
answer
Gamma- no hemolysis or discoloration of the media
Alpha - incomplete hemolysis or greening of the media
Beta - complete hemolysis or clearing of the media
question
What is the KOH test?
answer
Confirms Gram stain based on whether 3% KOH can lyse bacterial cell wall and release DNA
question
What is MSA?
answer
Selects for microorganisms based on their ability to grow with 7.5% NaCl present in the agar and then differentiates organisms based on their ability to ferment mannitol.
Procedure: Using a sterile loop, inoculate and streak the test organism for isolation onto a MSA plate.
question
What is the interpretation of MSA?
answer
Positive: Growth + / Acid +. Yellow colonies (Acid is produced by mannitol fermentation.)
Negative: Growth + / Acid - or Growth - / Acid -. Colorless colonies (no yellow color / no acid production) or no growth
question
What is a nitrate reduction?
answer
Differentiates microorganisms based on their ability to reduce nitrate to nitrite or ammonia or nitrogenous gas.
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question
What is the purpose of BHI with 6.5% NaCl
answer
Differentiates microorganisms based on their ability to grow in 6.5% NaCl
question
What is the purpose of bile esculin hydrolysis?
answer
Differentiates mnicroorganisms based on their ability to hydrolyze esculin to esculetin and dextrose. The esculetin reacts with ferric citrate in the medium to dorm a dark-brown complex.
question
What is catalase purpose?
answer
Differentiates microorganisms based on their ability to produce the catalase enzyme. Catalase breaks hydrogen peroxide down to water and oxygen.
question
What is coagulase's purpose?
answer

Detection of coagulase that is excreted by Staphylococcus aureus that causes fibrin clot formation.

question
What are the three types of hemolysis on SBA?
answer
Gamma- no hemolysis or discoloration of the media
Alpha - incomplete hemolysis or greening of the media
Beta - complete hemolysis or clearing of the media
question
What is the KOH test?
answer
Confirms Gram stain based on whether 3% KOH can lyse bacterial cell wall and release DNA
question
What is MSA?
answer
Selects for microorganisms based on their ability to grow with 7.5% NaCl present in the agar and then differentiates organisms based on their ability to ferment mannitol.
Procedure: Using a sterile loop, inoculate and streak the test organism for isolation onto a MSA plate.
question
What is the interpretation of MSA?
answer
Positive: Growth + / Acid +. Yellow colonies (Acid is produced by mannitol fermentation.)
Negative: Growth + / Acid - or Growth - / Acid -. Colorless colonies (no yellow color / no acid production) or no growth
question
What is a nitrate reduction?
answer
Differentiates microorganisms based on their ability to reduce nitrate to nitrite or ammonia or nitrogenous gas.
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