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Microbiology Lab – Final

question
partial hemolysis on blood agar plates is called:
answer
alpha
question
complete hemolysis on a blood agar plate is called:
answer
beta
question
no hemolysis on a blood agar plate is called:
answer
gamma
question
blood agar plates are _______ for hemolysis:
answer
differential
question
which type of media is NOT differential?
answer
phenylethanol agar
question
phenylethanol agar is used:
answer
to select for gram positive bacteria
question
T/F - macconkey (MAC) agar is enriched with salt
answer
false
question
eosin methylene blue (EMB) agar is selective for _________ and differential for __________
answer
gram negative organisms, lactose fermentation
question
mannitol salt agar (MSA) is selective for ___________ and differential for _____________
answer
mannitol fermentation, gram negative organisms
question
which of the following types of agar would be the most helpful for isolating and differentiating staphylococcus aureus from other types of organisms?
answer
mannitol salt agar
question
a ____________ medium contains one or more compounds to distinguish between microbes in the medium, while an ____________ medium contains one or more additives to encourage the growth of fastidious microbes
answer
differential, enriched
question
macconkey agar is differential for ____________ fermentation
answer
lactose
question
a durham tube is used to detect __________ produced by carbohydrate fermentation
answer
gas
question
how does alpha hemolysis appear on blood agar
answer
a green/brown zone surrounding the colony
question
the enzyme phenylalanase converts phenylalanine into
answer
ammonia and phenylpyruvic acid
question
T/F - a mannitol fermenter appears pink on mannitol salt agar
answer
false
question
SIM media detects the production of hydrogen sulfide, indole production, and motility. A positive indole test indicates the enzyme _____ was produced by the bacterial strain
answer
tryptophanase
question
carbohydrate fermentation:
answer
production of acid and/or gas
question
_______________ is the enzyme responsible for digesting starch
answer
amylase
question
the urease converts urea into carbon dioxide and ____________
answer
ammonia
question
a positive oxidase result:
answer
turns purple
question
what color will lactose fermenter appear on eosin methylene blue (EMB) agar?
answer
green metallic
question
T/F - to test for starch hydrolysis, the reagent iodine was added to the plate after incubation
answer
true
question
which of the following organisms is used as an indicator of fecal contamination of water?
answer
escherichia coli
question
the reagent used to test for phenylalanine deamination is __________; the reagent turns ______________ as a positive result
answer
ferric chloride, green
question
what is the term that is used if water is coliform free and drinkable?
answer
potable
question
which types of media did you use to isolate your unknown organisms?
answer
MAC and MSA
question
what is one illness caused by staphylococcus saprophyticus?
answer
UTI
question
which of the following organisms are NEVER part of the normal flora of the skin, mucous membranes, and throat?
answer
staphylococcus saprophyticus
question
a negative test for utilization of citrate would result in ______________
answer
a green color
question
T/F - shigella can cause typhoid fever
answer
false
question
which of the following is part of the definition for a coliform?
answer
ferments lactose to acid and gas, gram negative, facultative anaerobes
question
T/F - the only member in group b streptococcus is streptococcus agalactiae
answer
true
question
staphylococci are _____________ while streptococci are:
answer
catalase positive, catalase negative
question
what is one disease that streptococcus pyogenes can cause?
answer
strep throat
question
in lab, we tested streptococcus pneumoniae and streptococcus viridans for susceptibility to:
answer
optochin
question
what is one disease that streptococcus pneumoniae can cause?
answer
pneumonia
question
which group of streptococci is responsible for the formation of dental plaque?
answer
viridans group
question
which of the following organisms is a member of group D streptococcus?
answer
enterococcus faecalis
question
which of the following tests did you perform on your staphylococcus unknown last period?
answer
coagulase
question
which of the following tests did you perform on your enterobacteriaceae unknown last period?
answer
citrate and SIM
question
which of the following would be a positive result for the production of the enzyme coagulase?
answer
the formation of a clot in the test tube
question
T/F - the rapid staphylococcus aureus test is based on antigen/antibody reactions
answer
true
question
which of the following organisms produces tyrptophanase?
answer
escherichia coli
question
suppose you are given an unknown sample that you need to identify as either salmonella or shigella. to accomplish this, you decide to use the rapid ID test. when you added the shigella antiserum to your sample, white clumps were produced while the salmonella antiserum resulted in a cloudy sample with no clumps. based on these results, what was the identity of your unknown?
answer
shigella
question
which of the following organisms is resistant to the antibiotic novobiocin?
answer
staphylococcus saprophyticus
question
T/F - when trying to identify bacterium as a member of streptococcus group A, B, C, or D, testing for susceptibility to the antibiotic bacitracin will give you sufficient information to identify the organism
answer
false
question
which of the following organism causes beta hemolysis on blood agar?
answer
staphylococcus aureus
question
which of the following organisms ferments lactose?
answer
escherichia coli
question
what would a positive test for the rapid detection of streptococcus pyogenes look like?
answer
two pink lines
question
which of the following organisms utilizes citrate?
answer
salmonella
1 of 51
question
partial hemolysis on blood agar plates is called:
answer
alpha
question
complete hemolysis on a blood agar plate is called:
answer
beta
question
no hemolysis on a blood agar plate is called:
answer
gamma
question
blood agar plates are _______ for hemolysis:
answer
differential
question
which type of media is NOT differential?
answer
phenylethanol agar
question
phenylethanol agar is used:
answer
to select for gram positive bacteria
question
T/F - macconkey (MAC) agar is enriched with salt
answer
false
question
eosin methylene blue (EMB) agar is selective for _________ and differential for __________
answer
gram negative organisms, lactose fermentation
question
mannitol salt agar (MSA) is selective for ___________ and differential for _____________
answer
mannitol fermentation, gram negative organisms
question
which of the following types of agar would be the most helpful for isolating and differentiating staphylococcus aureus from other types of organisms?
answer
mannitol salt agar
question
a ____________ medium contains one or more compounds to distinguish between microbes in the medium, while an ____________ medium contains one or more additives to encourage the growth of fastidious microbes
answer
differential, enriched
question
macconkey agar is differential for ____________ fermentation
answer
lactose
question
a durham tube is used to detect __________ produced by carbohydrate fermentation
answer
gas
question
how does alpha hemolysis appear on blood agar
answer
a green/brown zone surrounding the colony
question
the enzyme phenylalanase converts phenylalanine into
answer
ammonia and phenylpyruvic acid
question
T/F - a mannitol fermenter appears pink on mannitol salt agar
answer
false
question
SIM media detects the production of hydrogen sulfide, indole production, and motility. A positive indole test indicates the enzyme _____ was produced by the bacterial strain
answer
tryptophanase
question
carbohydrate fermentation:
answer
production of acid and/or gas
question
_______________ is the enzyme responsible for digesting starch
answer
amylase
question
the urease converts urea into carbon dioxide and ____________
answer
ammonia
question
a positive oxidase result:
answer
turns purple
question
what color will lactose fermenter appear on eosin methylene blue (EMB) agar?
answer
green metallic
question
T/F - to test for starch hydrolysis, the reagent iodine was added to the plate after incubation
answer
true
question
which of the following organisms is used as an indicator of fecal contamination of water?
answer
escherichia coli
question
the reagent used to test for phenylalanine deamination is __________; the reagent turns ______________ as a positive result
answer
ferric chloride, green
question
what is the term that is used if water is coliform free and drinkable?
answer
potable
question
which types of media did you use to isolate your unknown organisms?
answer
MAC and MSA
question
what is one illness caused by staphylococcus saprophyticus?
answer
UTI
question
which of the following organisms are NEVER part of the normal flora of the skin, mucous membranes, and throat?
answer
staphylococcus saprophyticus
question
a negative test for utilization of citrate would result in ______________
answer
a green color
question
T/F - shigella can cause typhoid fever
answer
false
question
which of the following is part of the definition for a coliform?
answer
ferments lactose to acid and gas, gram negative, facultative anaerobes
question
T/F - the only member in group b streptococcus is streptococcus agalactiae
answer
true
question
staphylococci are _____________ while streptococci are:
answer
catalase positive, catalase negative
question
what is one disease that streptococcus pyogenes can cause?
answer
strep throat
question
in lab, we tested streptococcus pneumoniae and streptococcus viridans for susceptibility to:
answer
optochin
question
what is one disease that streptococcus pneumoniae can cause?
answer
pneumonia
question
which group of streptococci is responsible for the formation of dental plaque?
answer
viridans group
question
which of the following organisms is a member of group D streptococcus?
answer
enterococcus faecalis
question
which of the following tests did you perform on your staphylococcus unknown last period?
answer
coagulase
question
which of the following tests did you perform on your enterobacteriaceae unknown last period?
answer
citrate and SIM
question
which of the following would be a positive result for the production of the enzyme coagulase?
answer
the formation of a clot in the test tube
question
T/F - the rapid staphylococcus aureus test is based on antigen/antibody reactions
answer
true
question
which of the following organisms produces tyrptophanase?
answer
escherichia coli
question
suppose you are given an unknown sample that you need to identify as either salmonella or shigella. to accomplish this, you decide to use the rapid ID test. when you added the shigella antiserum to your sample, white clumps were produced while the salmonella antiserum resulted in a cloudy sample with no clumps. based on these results, what was the identity of your unknown?
answer
shigella
question
which of the following organisms is resistant to the antibiotic novobiocin?
answer
staphylococcus saprophyticus
question
T/F - when trying to identify bacterium as a member of streptococcus group A, B, C, or D, testing for susceptibility to the antibiotic bacitracin will give you sufficient information to identify the organism
answer
false
question
which of the following organism causes beta hemolysis on blood agar?
answer
staphylococcus aureus
question
which of the following organisms ferments lactose?
answer
escherichia coli
question
what would a positive test for the rapid detection of streptococcus pyogenes look like?
answer
two pink lines
question
which of the following organisms utilizes citrate?
answer
salmonella
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